Table I.

Comparison of published protocols used to dissociate mouse ovarian tissue.

Year	Timepoint	Mouse	Mincing of tissue?	Enzymes used	Cell types isolated	Filtration used?	Enzyme dissociation time	Reference
2012	Adult (8wk)	Wild-type C57BL6	Mince using a scalpel	800 U/ml Collagenase 0.001 mg/ml DNAse 1 0.05% trypsin-EDTA	All ovarian cells	70 µm	15min	White et al. (2012)
2013	Adult (6–8 weeks)	Wild-type—no specific strain	Mince using a scalpel	800 U/ml Collagenase 0.001 mg/ml DNAse 1	Oogonial stem cells	100 µm	30 min	Woods and Tilly, (2013)
2019	E10.5—E13.5, E16.5, PND6	Tg (Nr5a1-GFP) CD1 mice	No	Trypsin 0.05% EDTA	Somatic cells	40 µm	10 min	Stévant et al. (2019)
2019	PND2 and PND6	Wild-type F1 mice	No	0.05% Trypsin 0.53 mM EDTA 0.02% DNAse 1	All ovarian cells	No	30 min	Lo et al. (2019)
2020	E11.5, E12.5, E14.5, E16.5, E18.5, PND1 and PND5	Wild-type—no specific strain	No	0.25% Trypsin	All ovarian cells	70 µm then 30 µm	E11 and 12 20 min E14 and 16 40 min E18 60 min P1 and 5 80 min	Niu and Spradling, (2020)
2020	E16.5, P0, P6	Wild-type C57BL6	Cut into small pieces	0.25% Trypsin 2 mg/ml Collagenase	All ovarian cells	40 µm	6–8 min	Wang et al. (2020)
2021	E12.5, E13.5, E16.5, E18.5	Tg (Nr5a1-GFP) CD1 mice	No	Trypsin 0.05% EDTA	All ovarian cells	70 µm	15 min	Mayère et al. (2021)