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Papain dissociation collection characteristics by ovarian timepoint (N = 4).
| Age of ovary | Cell yield from one ovary, mean ± SD | Cell viability from one ovary, mean ± SD | RNA yield (ng RNA per 100,000 cells), mean ± SD | RIN score, mean ± SD |
|---|---|---|---|---|
| E18.5 | 6.49 ± 2.0 × 104 | 75% ± 0.1 | 81 ± 49 | 9.5 ± 0.9 |
| PND8 | 1.25 ± 0.7 × 105 | 85% ± 0.0 | 78 ± 25 | 9.7 ± 0.3 |
| 12WKS | 3.00 ± 1.0 × 105 | 67% ± 0.1 | 83 ± 43 | 9.5 ± 0.4 |
PND, post-natal day; RIN, RNA Integrity number; WKS, weeks.
