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. 2021 Jun 4;12:670216. doi: 10.3389/fpls.2021.670216

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Copyright © 2021 Zhu, Guo, Sun, Wang and Zhou.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Root inoculation with L. fusiformis Cr33 inhibited root Fe deficiency responses under Cd stress condition. Three-weeks-old tomato plants cultured in the split-root systems were inoculated with or without Cr33 for 3 d. Then, these plants were exposed to 100 μM Cd with or without Cr33 for 48 h. These plants were used to analyze root No content (A), the expression of Fer (B), IRT1 (C), FRO1 (D). In addition, root FCR activities were quantified (E) and assessed by observing purple color (F). Asterisks indicated significant differences between different experimental groups (n = 8 biological replicates) using Student’s t-test (**p < 0.01).