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. 2021 Jun 4;12:695009. doi: 10.3389/fphar.2021.695009

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Copyright © 2021 Noh, Zhang, Kim, Park, Kim and Kwon.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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PD specifically inhibited USP/UAF1 activity. (A) Schematic representation of Ub-AMC assay. USP1 removes ubiquitin from its substrate Ub-AMC, and fluorescent AMC is measured. (B) rUSP1/UAF1 complex or rUSP2 were incubated with control, USP1 inhibitor SJB2-043, ML-323, or PD for 30 min at 37°C, followed by assessment of DUB activity using Ub-AMC assay. (C) Progress curve for USP1/UAF1 activity on PD against Ub-AMC. The graph represents the average of three independent experiments with calculated SEM. The experiment was conducted at least four times.