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. 2021 Jun 4;12:695009. doi: 10.3389/fphar.2021.695009

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Copyright © 2021 Noh, Zhang, Kim, Park, Kim and Kwon.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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USP1 knockdown inhibits VEGF-induced endothelial permeability and stabilizes junctional proteins. (A) Expression of USP1 mRNA by RT-PCR following transfection with USP1 siRNA. (B) Western blot analysis shows USP1 expression in cells transfected with USP1-siRNA. Treatment with both VEGF and siUSP1 resulted in decreased endothelial permeability of TEER (C) and 70 kDa dextran (D) as compared with that obtained with VEGF treatment alone in HUVECs. n ≥ 3 independent experiments. siUSP1 cells were fixed, permeabilized, and subsequently immunostained for VE-cadherin (E) and F-actin (F). All data are presented as means ± SEM. *p < 0.05, ***p < 0.0005, ****p < 0.0001. Scale bar = 20 μM.