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. 2021 Jun 4;12:695009. doi: 10.3389/fphar.2021.695009

FIGURE 6.

FIGURE 6

USP1 inhibitor blocks VEGF-induced endothelial permeability and stabilizes junctional proteins. HUVECs were starved and treated with SJB2-043 (1 μM, 30 min) and ML-323 (5 μM, 30 min) before stimulation with VEGF (30 ng/ml, 30 min). SJB2-043 and ML-323 blocked both the TEER decline (A,B) (Experiments were performed in triplicate) and the increase in FITC-dextran transendothelial permeability (C,D) (Combined data from the six independent experiments are shown) induced by VEGF. HUVECs were treated with SJB2-043 (1 μM, 30 min) and ML-323 (5 μM, 30 min) before stimulation with VEGF (50 ng/ml, 30 min). Cells were fixed, permeabilized, and subsequently immunostained for VE-cadherin (E) and F-actin (F) All data are presented as means ± SEM, *p < 0.05, ***p < 0.0005, ****p < 0.0001 vs control group; #p < 0.05, ##p < 0.005, ###p < 0.0005, ####p < 0.0001 vs VEGF treated group. Scale bar = 20 μM.