FIGURE 5.

SA ameliorates oxidative stress, pyroptosis and reduces lipid accumulation in EtOH-treated AML-12 cells by regulating BRD4. (A) AML-12 cells were transfected with BRD4 siRNA or control siRNA for 24 h, 20 μM SA for 6 h, before exposure to EtOH (100 mM) for 24 h or not. Expression of BRD4 was measured in vitro (n = 3). (B) Western blotting of Nrf2, HO-1, and SOD2 levels (n = 3). (C) Western blotting of NLRP3, GSDMD, activated caspase-1, IL-18 and IL-1β protein levels (n = 3). *p < 0.05, **p < 0.01 vs the control group; ^# p < 0.05, ^## p < 0.01 vs the EtOH group. (D) Intracellular lipid accumulation was measured by Nile Red staining (×200). Scale bar = 50 μm. (E) DCFH-DA staining (×200). Scale bar = 50 μm.