FIGURE 3.

Reversal of β-lactam resistance in MRSA strain COL by addition of hit-compounds. The sensitivity of the COL MRSA strain towards different β-lactams and vancomycin (negative control) in the absence or presence of hit-compounds was examined by a disc diffusion assay. Results of the disc diffusion assay performed with four compounds with decreasing ability to increase the growth yield of S. aureus clpX mutants are shown. The hit-compounds were added to the agar at the indicated sub-lethal concentrations, and antibiotic susceptibility discs were placed on a lawn of the MRSA strain COL as indicated on the left diagram.