Table 2.

Internalization of UC-961 and VLS-101 into tumor cells

Time, h	% ROR1 cell-surface receptor relative to time 0
	UC-961, mean ± SD		VLS-101, mean ± SD
	Jeko-1	Primary CLL	Jeko-1		Primary CLL
0	100.0 ± 0	100.0 ± 0	100.0 ± 0		100 ± 0
0.5	60.7 ± 9.3	40.5 ± 7.0	54.1 ± 1.9		37.7 ± 4.5
1	53.0 ± 5.6	35.0 ± 4.2	48.7 ± 4.7		30.7 ± 1.5
2	45.0 ± 5.0	29.0 ± 3.1	42.2 ± 6.4		26.0 ± 1.7
4	23.3 ± 7.2	12.0 ± 1.5	22.7 ± 7.7		10.0 ± 3.6

JeKo-1 or CLL cells were harvested and incubated at 4°C with saturating concentrations of UC-961 or VLS-101 (60 µg/mL), washed, and then incubated at 37°C for the indicated times (h). After incubation, cells were washed and stained with a fluorophore-conjugated secondary antibody and then analyzed by flow cytometry. The degree of receptor internalization was determined using the amount of UC-961 or VLS-101 present at each time point when compared with the amount of receptor present at time 0 in 2 independent experiments for the JeKo-1 cells and 3 different primary CLL patient samples.

SD, standard deviation.