Fig. 4.

Circ-SPG11 sponged miR-337-3p and negatively regulated miR-337-3p expression. A Circinteractome was used to predict the underlying binding sites between circ-SPG11 and miR-337-3p. B, C Dual-luciferase reporter system and RIP assay were employed to assess the combination between circ-SPG11 and miR-337-3p. D QRT-PCR was devoted to testing miR-337-3p expression in OA tissues and normal tissues. E The relationship between the expression of circ-SPG11 and miR-337-3p was analyzed by Pearson correlation coefficient. F MiR-337-3p expression was examined by qRT-PCR in CHON-001 cells treated by 0 ng/mL, 5 ng/mL, and 10 ng/mL, 15 ng/mL of IL-1β for 24 h. G MiR-337-3p expression was examined by qRT-PCR in CHON-001 cells treated by 10 ng/mL of IL-1β for 0 h, 12 h, 24 h, and 48 h. H QRT-PCR was used to examine circ-SPG11 expression in CHON-001 cells without treatment or treated with IL-1β and transfected with circ-SPG11 or negative control. I MiR-337-3p expression was detected by qRT-PCR in CHON-001 cells without treatment or treated with IL-1β and transfected with circ-SPG11 or negative control. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001