Fig. 2.

Filtering criteria applied to raw variant calls to identify potential off-target mutations and spontaneous de novo mutations in the gene-edited cell line CC14. The white boxes adjacent the filtering criteria indicate the number of candidate mutations remaining after the filter was applied. *Variants were kept if also present in 1805 and/or B071 **Predicted heterozygous de novo mutations were also filtered for their presence in calves 1805 and B071