Figure 3.

Concentration dependence of inhibition of hGLUT‐1 mediated [³H]2‐DG uptake by TKIs. Effects of increasing concentrations of bosutinib, gefitinib, pazopanib, and temsirolimus, respectively, on uptake of [³H]2‐DG in FaDu cells are shown in panels (a–d). Effects of 0 (○), 25 (●), 50 (∆), or 100 (▲) µM of bosutinib, gefitinib, pazopanib, or temsirolimus, respectively, on [³H]2‐DG uptake rates are shown in panels (e–h) as Lineweaver–Burk transformations of the data. Each experiment was repeated three times with three to four replicates for each experiment and values (means ± SE) are shown in each panel. 2‐DG, 2‐deoxy‐d‐glucose; hGLUT, human glucose transporter; TKI, tyrosine kinase inhibitor.