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. Author manuscript; available in PMC: 2021 Jun 18.
Published in final edited form as: Nat Protoc. 2021 Jan 11;16(2):919–936. doi: 10.1038/s41596-020-00441-w

Fig. 1 |. Overview of the protocol.

Fig. 1 |

Schematic representation of the protocol from passage of PSCs to final HBPO. The image brightness was increased to improve visibility. Refer to Table 1 for the specific details of the different culture media that are required at each stage of the protocol. dFBS, defined FBS.