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. Author manuscript; available in PMC: 2021 Jun 18.
Published in final edited form as: Nat Protoc. 2021 Jan 11;16(2):919–936. doi: 10.1038/s41596-020-00441-w

Table 1 |.

Reagent reconstitution

Solvent Stock concentration
Activin A 4 mM HCl, PBS 100 μg/ml
BMP4 0.1% (wt/vol) BSA, 4 mM HCl, PBS 50 μg/ml
Noggin 0.1% (wt/vol) BSA, PBS 200 μg/ml
FGF4 0.1% (wt/vol) BSA, PBS 100 μg/ml
CHIR99021 Dimethyl sulfoxide 20 mM
Y-27632 PBS 10 mM