Figure 5.

Combined administration of PAI and Sal reduces the growth and metastasis of the murine OS Allograft model. (A) C3H mice were subcutaneously inoculated with LM8 cells (2 × 10⁶) at day 0 and intravenously administered 200 μL of PAI (50%) and Sal (3 mg/Kg) alone or in combination 3 times per week for 4 weeks from day 7. The sizes of the tumors in the mice were measured every week. Values represent the mean ± SD (n = 6). *P < 0.05 compared with the corresponding control. ^## P < 0.01 vs. PAI or Sal alone. (B) Representative photos of primary tumors at 5 weeks after inoculation of LM8 cells. Bar = 1 cm. Simultaneously, lungs were removed, and metastatic nodules on their surface were counted with a stereomicroscope. (C) Representative microscope image of lungs (hematoxylin-eosin staining). Bar = 200 μm. (D) Quantitative analysis of metastatic nodules in the lungs. Data were analyzed by one-way analysis of variance followed by Tukey’s post hoc test. Results are shown as mean ± SD. *P < 0.05 compared with the corresponding control. ^# P < 0.05 vs. PAI or Sal alone. (E) The weights of the mice were measured every week for 5 weeks. Data are the mean ± SD (n = 6). See Supplementary Figure 7 schematic flowchart and photos of the tumor-bearing mouse.