Figure 2.

BM LSK cells in DB mice are intrinsically modified towards myeloid lineage commitment. (A, B) Gene expression in LSK cells: (A) experimental design and (B) RT-qPCR for mRNA expression for transcription factors and surface receptors associated with myeloid commitment in LSK cells isolated from DB and ND BM. Data shown as relative fold-change of mRNA transcript in DB mice as compared with ND controls. (C, D) Surface M-CSFR protein: (C) experimental design and (D) representative flow cytograms (upper panel) and percentage (of LSK cells) and mean fluorescence intensity (MFI) of M-CSFR on LSK cells in DB and ND BM. Mean ± SD, n=4–5 (A, B) and 7 (C, D) mice for each strain. One sample was excluded from statistical analysis for Spi1 and Cebpa mRNA expression (in B) because of very high PCR cycle numbers and thus we considered this data point an outlier. Data were compared between DB and ND groups using a Mann–Whitney U-test. Differences between groups were considered significant if p≤0.05. ^*p≤0.05; ^**p≤0.01; ***p<0.001.