T2D increases Ly6Chi Mo numbers in the circulation and spleen and Mo/Mp in the wounds of DB mice following skin wounding. (A–C) BM, circulating and splenic Ly6Chi Mo; (A) percentage (of total cells) of Ly6Chi Mo in BM, (B) PB and (C) spleen of DB and ND mice at different time points following skin wounding. (D–G) Wound Mo/Mp; (D) representative flow cytograms with mean percentages, and absolute numbers of (E) Ly6Chi Mo, (F) Ly6Chi Mp, and (G) Ly6Clo Mp in the wounds of DB and ND mice at different time points post-skin wounding. Mean±SD, n=5–6 mice for each time point for each strain. Data were compared between DB and ND groups over different time points using two-way ANOVA and over different time points in the same group using one-way ANOVA (Kruskal–Wallis test) in kinetic assays. Differences between groups were considered significant if p≤0.05. a, mean value significantly different from day 0 in ND; b, mean value significantly different from day 0 in DB; c, mean value significantly different between two strains at the same time point.