TABLE 2.

Primer pairs of the novel mutations of paired box 3 (PAX3), SRY-box transcription factor 10 (SOX10), and melanocyte inducing transcription factor (MITF).

Mutations	Affected family	Forward primer sequence (5′–3′)	Reverse primer sequence (5′–3′)	Product length
PAX3:c.1459C > T	WS01	GCCCAAACCAGTCTGGGTAAAT	GCATGACCTAAAAAGCTGCGT	471 bp
PAX3:c.123del	WS02	AGGACGTATGGAGCCAGTCT	GAGTCCGATGTCGAGCAGTT	351 bp
SOX10:c.198_262del	WS03	TGGTCTTCCAGCCCTATCCA	CAGGCGAGCTGGGCAAG	419 bp
SOX10:c.529_556del	WS04	CAGGGTCTCATTGCCATCCA	CAGGGCCTCACATCTTCCAA	459 bp
MITF:c.731G > A	WS05	GCAAACACTCGTGAATGGCA	CTGAGCAACAAATGCCGGTT	510 bp
MITF:c.970dup	WS06	TTCCCTTATTCCATCCACGGG	TCAGTCCCAGTTCCGAGGTT	186 bp
PAX3:c.959-409_1173+3402del	WS07	GAGCGCGTAATCAGTCTGGG	GGCCACATTTAGGACATGCG	19,658/15,633 bp*
	WS07	AAAATGCACAGACCCTTTCAGCA	TCTGGTTTAGCAACCGCCG	4,998/973 bp*

*The product length of the normal allele and mutated allele is separated by the slash. Reference sequence transcript: PAX3: NM_181459.4; SOX10: NM_006941.4; and MITF: NM_000248.3.