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Growth activity of SPY131 (emm1), SPY128 (emm12), their ssa mutants (∆ssa), covS mutants (∆covS), and covS and ssa double mutants (∆covS∆ssa) in human whole blood. Group A Streptococcus (GAS) strains were incubated with whole blood from donors for 1.5 h. The number of surviving bacteria in human blood was determined by plating and enumerating the colony forming units (CFUs), which determined growth relative to the initial inoculum. *p < 0.05.
