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. 2021 Jun 4;12:685343. doi: 10.3389/fmicb.2021.685343

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Copyright © 2021 Chiang-Ni, Liu, Lin, Hsu, Shi, Chen, Lai and Chiu.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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PCR and Southern blot analyses of the ssa mutant and lysogenic convertants. (A) Detection of the chloramphenicol cassette (cm) and the chromosomal speB gene in the chromosomal DNA and filtrated supernatant after mitomycin C treatment using PCR. SCN279, the phage donor. A20 (emm1-type), the recipient. (B) Southern blot hybridization of lysogenic convertants. The upper panel shows the HindIII site and the predicted sizes for cm probe hybridization according to ΦHKU.vir (HKU360, NCBI accession no. CP009612) and A20 (NCBI accession no. CP003901.1) sequence. The recipient strains (A20, CovS_H280A, and ∆covS) showed no signal for cm probe hybridization and used as the experimental negative control. M, DNA marker.