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Reproducibility of reverse transcriptase quantitative polymerase chain reaction (RT-qPCR).
| Mean CT values ± standard deviation (n = 5) | ||
|---|---|---|
| Concentration of pMD19-BRBV (copies/μl) | Intra-assay | Inter-assay |
| 2.23 ×102 | 31.52 ± 0.36 | 31.17 ± 0.16 |
| 2.23 ×104 | 24.36 ± 0.43 | 24.54 ± 0.97 |
| 2.23 ×106 | 15.50 ± 0.55 | 14.51 ± 0.38 |
