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. 2021 Jun 4;12:681615. doi: 10.3389/fimmu.2021.681615

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Copyright © 2021 Ziblat, Iraolagoitia, Nuñez, Torres, Secchiari, Sierra, Spallanzani, Rovegno, Secin, Fuertes, Domaica and Zwirner

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Normalization of the expression of CD85j, CD45 and CD48 on NK cells significantly discriminates between ccRCC and HD samples. Normalization of the expression of CD85j, CD45 and CD48 in PBNK was performed calculating the respective z-scores and individual z-scores for CD85j (A), CD45 (B) and CD48 (C) were depicted. Also, the sum of z-scores was calculated for the pairs of receptors CD85j and CD45 (D), CD85j and CD48 (E), and CD45 and CD48 (F) as well as the sum of z-scores of CD85j, CD45 and CD48 (G). The dashed line in each graph indicates the value of the mean plus 3 SD. For HD: n=10 (A), n=12 (B), n=11 (C, F), n=9 (D) and n=8 (E, G). For P: n=10 (A, D), n=12 (B) and n=9 (C, E–G). *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001.