Figure 3.

FHL3 knockdown significantly sensitized the efficacy of OHP treatment in gastric cancer. (1) The gastric cancer cells were screened for FHL3-high-expression cell lines by western blot (A); (2) MMT was performed to detect the growth of OHP-resistance cell lines when treated with NS or OHP for 5 days in HGC and SGC cell lines (B, C); (3) The knockdown efficiency of the four FHL3 knockdown sequences in OHP-resistance cell lines, through western blot and immunofluorescence analysis (100× magnification); we found that FHL3-SH1 was the most efficient sequence for down-regulation of FHL3 (D–F); (4) OHP-resistance FHL3-NC and SH1 cell lines were treated with OHP to detect the proliferation via colony formation (G, I, J); (5) OHP-resistance FHL3-NC and SH1 cell lines were treated with OHP to detect the efficacy of OHP via colony formation and live and death staining assay (200× magnification, H, K and L).