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. 2021 Jun 4;11:649029. doi: 10.3389/fonc.2021.649029

Figure 7.

Figure 7

The genetic characteristics and pathways’ activation by FHL3 knockdown. (1) In KEEG and GO analyses, MAPK, PI3K/Akt and TGFβ pathways are related to FHL3 (A, B); (2) The expression and activation levels of MAPK pathway proteins were detected by western blot: down-regulation of ERK1/2, Thr202/204-ERK1/2 (p-ERK1/2), p-ERK1/2/ERK1/2 and Thr180/182-P38 and p-P38/P38 by FHL3 knockdown was observed (C1–4); (3) The expression and activation levels of PI3K pathway proteins were detected: down-regulation of PI3K-p85, Tyr607-PI3K-p85 (p-PI3K), S473-Akt (p-Akt), p-Akt/Akt, Ser9-GSK3β and Ser9-GSK3β/GSK3β, and up-regulation of Akt, Ser217/279-GSK3β and Ser217/279-GSK3β/GSK3β by FHL3 knockdown were observed (D1–4); (4) The expression and activation levels of TGFβ pathway proteins were detected: down-regulation of TGFβ, Thr8-Smad2/3 (p-Smad2/3), Ser423/425-Smad2/3 (p-Smad2/3), and p-Smad2/3/Smad2/3 by FHL3 knockdown were observed, while the status of Smad4 was not significantly affected by FHL3 (E1–4).