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31P NMR chemical shifts in d8-toluene plotted as a function of temperature for the AD 2-mer (green), a 1:1 mixture of AA and DD (red), and a 1:1 mixture of A and D (blue). The concentration of AD was 6 mM, and the concentrations of AA, DD, A, and D were 3 mM, so that the concentration of fully bound duplex was the same in all experiments.
