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. 2021 Jun 8;17(6):e1009071. doi: 10.1371/journal.pcbi.1009071

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© 2021 Burton et al

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Fig 6 — Meta-clustering results for FlowSOM (top) and Phenograph (bottom) when applied to blood T cells after batch effect correction with Harmony. Heatmaps show the normalised expression of cell surface markers for meta-clusters (clustered centroids of individually clustered patient samples). In the neighbouring UMAP plots, clusters from all patients are shown in the same embedded space and coloured by their meta-cluster membership. The size of each data point corresponds to the percentage of T cells this cluster represents in the patient it was derived from.