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. 2021 Jun 18;16(6):e0252547. doi: 10.1371/journal.pone.0252547

Fig 2. PD-1+ T cells observed in aged mice are memory-phenotype (MP) and express senescence marker, p21.

Fig 2

(A) Spleen cells from 80-week-old mice were stained for CD3, CD4, CD8, CD44, and CD62L, and then analyzed by flow cytometry. Results indicated that PD-1+ T cells are mainly MP because they expressed higher levels of CD44 and lower levels of CD62L. (B, C) Flow cytometric analysis of p21 expression in PD-1+ CD44+ CD4+ T cells and PD-1- CD44+ CD4+ T cells obtained from eVAT and the spleen of 80-week-old mice (n = 5–6 mice in each group). (D) Flow cytometric analysis of H2AX expression in PD-1+ CD3+ T cells and PD-1- CD3+ T cells obtained from eVAT and the spleen of either 11-week-old or 80-week-old mice (n = 5–6 mice in each group). *P < 0.05; NS: not significant. Data are represented as the mean ± SEM.