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. 2021 Jun 4;12:643509. doi: 10.3389/fmicb.2021.643509

TABLE 1.

Oligonucleotide primers used for the molecular identification of dermatophytes.

Locus Primers Sequence (5′–3′) Annealing temperature (°C) References
TEF-1α Forward CACATTAACTTGGTC GTTATCG 58 Mirhendi et al., 2014
Reverse CATCCTTGGAGAT ACCAGC
BT2 Forward AACATGCGTGAGA TTGTAAGT 56 Rezaei-Matehkolaei et al., 2014
Reverse ACCCTCAGTGTAGT GACCCTTGGC
CaM Forward TGTCCGAGTACA AGGAAGC 60 Ahmadi et al., 2016
Reverse TTACAATCAAT TCTGCCGTC
ACT Forward TCTTCGAGACC TTCAACGCC 67 Probst et al., 2002
Reverse AAGCCACCGATC CAGACG
HSP70 Forward GTGGCTTCCCA GGTGCTG 55 Probst et al., 2002
Reverse AATGATTTCAGTAAC CGACCC
ITS Forward TCCGTAGGTGAACC TGCGG 58 White et al., 1990
Reverse TCCTCCGCTTATTG ATATGC
D1/D2 Forward GCATATCAATAAGCGGA GGAAAAG 60 Kurtzman and Robnett, 1997
Reverse GGTCCGTGTTTCA AGACGG