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. 2021 Jun 4;12:690681. doi: 10.3389/fendo.2021.690681

Figure 6.

Figure 6

Genetic ablation of PC5 alone or in combination with Furin does not impair FGF23 processing in vivo. Plasma intact FGF23 (A) and serum phosphate levels (B) in 4-month-old Pcsk5flox/flox (n=6) and Pcsk5osb-/- (n=7) mice fed a normal chow diet. (C) Von Kossa/van Gieson staining of vertebrae sections of Pcsk5flox/flox and Pcsk5osb-/-. (D) Plasma intact FGF23 in 6-month-old Furin;Pcsk5flox/flox (n=7-8) and Furin;Pcsk5osb-/- (n=8) fed a normal phosphate diet (0.6%) or a low phosphate diet (0.02%) for one week. (E, H) Plasma FGF23 measurements in 4-month-old Furin;Pcsk5flox/flox (n=4) and Furin;Pcsk5osb-/- (n=4) 6 hours following an injection of vehicle and 300 U/kg of recombinant human erythropoietin (rhEPO): C-terminal FGF23 (E), intact FGF23 (F), percentage (%) of intact over C-terminal FGF23 (G) and cleaved FGF23 levels (H), calculated as (C-terminal FGF23) - (intact FGF23). Results represent the mean ± SEM. *P < 0.01, **P < 0.01 and ***P < 0.001, by 2-way ANOVA with Bonferroni’s multiple comparisons test, or by Student’s t-test (A, B).