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. 2021 Jun 16;8(3):ENEURO.0033-21.2021. doi: 10.1523/ENEURO.0033-21.2021

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Copyright © 2021 Carrette et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license, which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

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Figure 4. — Application example looking at N/OFQ levels in oxycodone biobank brain punches from rats with different addiction profiles. A, Immunoluminescent Western blottings that show nociceptin levels in naive, LA, and HA rats; n = 21 (n = 7 per group). B, Western blot analysis revealed a significant decrease in nociceptin levels (internally normalized to β-tubulin and a naive rat on the blot) in HA rats compared with naive rats; *p = 0.02 one-way ANOVA, error bars represent standard error of mean. C, Correlation between AI and the Z-score of nociceptin levels in the CeA; *p < 0.02 pearson correlation. Partial figure reproduced from Kallupi et al. (2020).