Skip to main content
. 2021 Jun 1;36(7):1891–1906. doi: 10.1093/humrep/deab135

Figure 6.

Figure 6.

The effect of PI3K and MEK inhibitors on FSH glycoform-dependent regulation of apoptotic and proliferative gene expression in ovarian wedge sections. Ovary sections were pre-treated with either media containing vehicle (Control), 10 μM U0126 (MEK inhibitor) (a–c) or 100 nM Wortmannin (Wort, PI3K inhibitor) (d–f) for 60 min. Following pre-treatment, ovarian sections were treated with media alone (Control), 30 ng/ml FSH18/21 or FSH24. Ovary wedge sections were snap-frozen and analyzed by quantitative RT-PCR. (a and d) Proliferating cell nuclear antigen (Pcna) gene expression is increased in ovary wedge sections treated with FSH18/21. (b and e) Bcl2/Bax ratio of ovary wedge sections, a high Bcl2 to Bax ratio is indicative of follicle health as seen in the FSH18/21 treatment group. (c and f) Apoptosis related expression of Caspase3 transcripts is increased following treatment with Wortmannin and U0126. Ovarian sections treated with FSH18/21 expressed reduced levels of Caspase 3 transcripts compared to sections treated with FSH24. Data was analyzed using one-way ANOVA with Dunnett’s post hoc test. Bars represent means ± SEM for six individual ovarian wedge sections. Bars marked a indicate a significant difference from Control (open bar). Comparisons among indicated treatments, *P < 0.05, **P < 0.01, ***P < 0.001.