Fig. 5.

RDN reduced the release of neutrophil extracellular traps. (A) Mice were intraperitoneally administered by Cl-amidine (20 mg/kg) and GSK484 (4 mg/kg) for day 0. The animals were euthanized 24 h after LPS instillation. H&E staining of lung sections from each group, respectively. Scale bar, 100 μm. Concentration of MPO (B) and NE (C) in BALF, serum and lung tissue were determined by ELISA. (D) Immunofluorescence staining of lung tissue sections from mice was performed, with imaging for cit H3 (green) and myeloperoxidase (MPO; red) and staining with DAPI for DNA-blue. Scale bar, 50 μm. (E) Immunoblot analysis of the PAD4 protein levels from lung tissues of each group. Data are expressed as the mean ± SEM of 5-6 mice. * p < 0.05, ** p < 0.01 vs. the LPS group.