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RDN injection reduced MAPK pathway in vitro. (A) Human neutrophils were stimulated with PMA (50 nM) for 3 hours. After 3 h, supernatants were collected (B) and the level of MPO was measured by using ELISA. (C) Western blot analysis of ERK 1/2, ERK 1/2 phosphorylation and PAD4. (D) The quantitative results of Western blot were shown. Data are shown as the mean ± SEM of three independent experiments. * p < 0.05, ** p < 0.01 vs. the PMA group.
