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. 2021 Jun 17;85:110064. doi: 10.1016/j.cellsig.2021.110064

Fig. 4.

Fig. 4

Effect of TRIM28 on IFN-γ induced ACE2 expression in A549 cells. (A, B) Control and TRIM28 silenced A549 cells were stimulated with IFN-γ for 16 h. qPCR assay was performed to check the expressions of ACE2 (A) and TRIM28 (B). n = 3. **P < 0.01. *P < 0.05. (C) The mRNA levels of IFNGR1 and IFNGR2 in control and TRIM28 knockdown A549 cells were examined. n = 3. **P < 0.01. (D) The protein levels of IFNGR2 in control or TRIM28 silenced cells were detected by western blot. n = 3. (E) Control or TRIM28 knockdown A549 cells were treated with IFN-γ for 15 min. Immunoblot analysis was performed to detect expressions of phosphorylated STAT1 and total STAT1. Representative blots were shown. n = 3. (F) A549 cells were transfected with scramble or IFNGR2 siRNA, and then treated with IFN-γ (20 ng/mL) for 16 h. ACE2 and IFNGR2 expressions were examined by qPCR method. n = 3. *P < 0.05.