Fig. 1.

OsJAZ11 is induced by Pi deficiency. a Expression pattern of OsJAZ11 in shoot (PB1.S) and root (PB1.R) of low-Pi-sensitive rice genotype PB1 under +P and −P conditions. Signal intensities were retrieved from previous microarray dataset (Mehra et al. 2016) submitted in Gene Expression Omnibus (GEO) database (accession no. GSE74795). b RT-qPCR of OsJAZ11 in roots and shoots of 30-day-old PB1 plants under +P (320 μM NaH₂PO₄) and −P (1 μM NaH₂PO₄) conditions. Each bar is the mean of three independent replicates with standard error. Significant differences between +P vs −P treatments were evaluated by Student’s t test. Asterisks * and *** indicate P values, ≤ 0.05 and 0.001, respectively (n = 3). c EMSA assays showing physical interaction of OsJAZ11 promoter with OsPHR2-6XHIS at P1BS cis-element. Interactions were performed using 50 ng of DIG-labeled OsJAZ11 promoter probe (− 1057 to − 1028 bp) flanking P1BS (lanes 1–6). Interactions were carried out with different concentrations of OsPHR2-6XHIS (100 ng, 200 ng, 300 ng and 400 ng in lane 2, 3, 4 and 5, respectively). In lane 6, for competition assays, 200 ng of OsPHR2-6XHIS and 200-fold excess of unlabeled OsJAZ11 promoter was used. In lanes 7–10, 50 ng of OsJAZ11 promoter probe was used with mutations in P1BS element (mP1BS). 100 ng, 200 ng, 300 ng and 400 ng of OsPHR2-6XHIS was used in lanes 7, 8, 9 and 10, respectively