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. 2021 Jun 18;11:12896. doi: 10.1038/s41598-021-92377-8

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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(a) Electrophoregram of bilirubin-10-sulfonate (ranarubin) and biliverdin-10-sulfonate (ranaverdin) mixture. Electrophoresis performed in background electrolyte 4 (see Table 1a). Bilirubin-10-sulfonate concentration = 0.1 g/L. eof electroosmotic flow rate marker. (b) Stability of bilirubin-10-sulfonate. Measured in background electrolyte 5 (see Table 1a). Bilirubin-10-sulfonate concentration = 0.1 g/L. (c) Dependence of effective electrophoretic mobility of bilirubin-10-sulfonate on sample concentration. Background electrolyte 12 (see Table 1a). Sample: bilirubin-10-sulfonate 0.1, 0.05, 0.01, 0.005 g/L in deionized water (5% DMSO). (d) Dependence of effective electrophoretic mobility and pK_a value of bilirubin-10-sulfonate on the content of DMSO. Background electrolyte 4 (see Table 1a).