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. 2021 Jun 18;12:3734. doi: 10.1038/s41467-021-24099-4

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — a hnRNPM was co-pulled down with the NCOR2 pre-mRNA using antisense probes directed to the hnRNPM-binding sites (hnRNPM-BSs) at intron 45 in A549 and H1299 cells as shown in RNA pulldown assays. Data are representatives of three independent experiments. S, sense; AS, antisense. b The hnRNPM-BSs at intron 45 of the NCOR2 pre-mRNA was co-precipitated with hnRNPM in A549 and H1299 cells using RIP assays. Data are representatives of three independent experiments. c Induced knockdown of PLANE decreased the amount of hnRNPM associated with the hnRNPM-BSs at the NCOR2 pre-mRNA, which was reversed by co-overexpression of a shRNA-resistant PLANE mutant (PLANE-R) as shown in RIP assays. Data are representatives or mean ± s.d.; n = 3 independent experiments, one-way ANOVA followed by Tukey’s multiple comparison test. d SiRNA knockdown of hnRNPM enhanced the NCOR2-202-generating AS event in A549 cells. Data are representatives of three independent experiments. e Relative levels of NCOR2-202/017 and NCOR2-001/005-like mRNA variants in cells with or without hnRNPM knockdown as shown in (d). Data are mean ± s.d.; n = 3 independent experiments, one-way ANOVA followed by Tukey’s multiple comparison test. f Overexpression of hnRNPM reduced the NCOR2-202-generating AS event, which was reversed by co-knockdown of PLANE in A549 cells. Data shown are representatives of 3 independent experiments. g Relative levels of NCOR2-202/-001/-005 as shown in (f) quantitated using densitometry. Data are mean ± s.d.; n = 3 independent experiments, one-way ANOVA followed by Tukey’s multiple comparison test. h Introduction of a shRNA-resistant PLANE mutant (PLANE-R) but not a PLANE mutant with its fragment 331–751 deleted (PLANE-R-Δ331-751) or its DFO deleted (PLANE-R-∆DFO) induced the association between hnRNPM and the hnRNPM-BSs at the NCOR2 pre-mRNA in A549.shPLANE cells with induced knockdown of PLANE. Data are representatives or mean ± s.d.; n = 3 independent experiments, one-way ANOVA followed by Tukey’s multiple comparison test. Source data are provided as a Source data file.