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. 2021 Jun 18;11:12903. doi: 10.1038/s41598-021-92303-y

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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COL4 and MYH11 immunohistochemical staining of non-lesioned ascending aorta and two examples of AAA lesions. To assist assessment of AAA lesions, the partially degraded medial (M) and adventitial (A) layers are labeled on overview images of COL4 stainings, and the media borders are marked with cyan/dotted lines. Images are representative specimens of 5 aortic punch- and 5 AAA-samples analyzed, and images of remaining specimens are shown in Supplementary Fig. 5. Scalebars (overview) = 1 mm; Scalebars (magnification) = 200 µm. AP = Aortic punch (ascending thoracic aorta). #1–#2 refer to sample IDs.