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. 2021 Jun 18;11:12880. doi: 10.1038/s41598-021-91974-x

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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MSS/MSI calling at locus level and integrative panel-based result for Idylla MSI assay, PCR and NGS versus immunohistochemistry. Samples are grouped according to colorectal (CRC) or uterine corpus endometrial carcinoma (UCEC) tumor type and MLH1/PMS2/MSH2/MSH6 immunohistochemical expression pattern with from top to bottom: pMMR CRC (n = 12), dMMR CRC (n = 16), pMMR UCEC (n = 9) and dMMR UCEC (n = 12) samples with the indicated tumor cell percentage, IHC pattern and case number. From left to right the results are shown of MSI calling in the 7, 8 and 10 loci of the Idylla MSI test, PCR and NGS-respectively. For each molecular technique the overall integrative binary scoring as microsatellite stable (MSS, blue) or microsatellite unstable (MSI, MSI-L: MSI-low; MSI-H: MSI-high, red) is listed first, followed by the scoring of the individual loci. Right columns indicate the presence (red, POS: positive) or absence (blue, NEG: negative) of pathogenic variants in NRAS/KRAS and BRAF in CRC and MLH1 promoter hypermethylation and (likely) pathogenic germline variants in MMR genes (MLH1, MSH2, MSH6, PMS2) in UCEC. Blank fields indicates parameter not assessed. Details on identified variants in supplementary Table S1.