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. 2021 Jun 18;12(7):627. doi: 10.1038/s41419-021-03917-z

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — A Schematic illustration showing the overlap among 13 miRNA target mRNAs in three databases. B KEGG analysis of 462 predicted target genes. C Putative binding site of miR-2110 with AFAP1-AS1 3′ UTR. D The luciferase activity of pmirGLO/lncRNA–AFAP1-AS1-WT/MUT plasmid in MDA–MB-231 and MDA–MB-468 cells after co-transfection with miR-2110. E Relative miR-2110 expression with shlncRNA–AFAP1-AS1 or NC. Unpaired student’s t-test and one-way ANOVA test were used for the statistical analyses. *P < 0.05; **P < 0.01; ***P < 0.005; ****P < 0.001; ns not significant.