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. 2021 Jun 18;12(7):627. doi: 10.1038/s41419-021-03917-z

Fig. 3. AFAP1-AS1 upregulated Sp1 level via sponging miR-2110.

Fig. 3

A Putative binding site of miR-2110 with Sp1 3′ UTR. B MiR-2110 expression in MDA–MB-231 and MDA–MB-468 cells were examined through RT-qPCR. C Relative protein level of Sp1 after miR-2110 mimics or inhibitor treatment. D The luciferase activity of pmirGLO/Sp1-WT/MUT plasmid after co-transfection with miR-2110. E The relative mRNA level of Sp1 after miR-2110 mimics or inhibitor treatment. F, G Relative protein and mRNA level of Sp1 after shlncRNA–AFAP1-AS1, miR-2110 mimics, and inhibitor treatment. Unpaired student’s t-test and one-way ANOVA test were used for the statistical analyses. *P < 0.05; **P < 0.01; ***P < 0.005; ****P < 0.001; ns not significant.