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. 2021 Jun 18;12:3797. doi: 10.1038/s41467-021-23824-3

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — A transgene derived from the endogenous Taar4 locus is not sufficient to drive reporter expression. a Schematic of the transgene backbone in which the Taar4 coding sequence in exon 2 (Ex. 2) is replaced with that of YFP (green box). Non-coding regions shown in gray. Defined transcription start site (TSS) is indicated (arrow). b Confocal image showing a wholemount of the olfactory turbinates of a ΔT4-YFPtg mouse showing lack of labeled OSNs. c Confocal image showing a wholemount of the dorsal olfactory bulb showing no labeled axons. d Diagram of the 5xHD-ΔT4-YFPtg, in which a strong OR enhancer (5xHD; black circle) is placed upstream of the putative promoter. e Wholemount fluorescence image of the olfactory turbinates showing robust expression of YFP in OSNs primarily in the dorsal olfactory epithelium. f YFP+ axons of OSNs primarily innervate the dorsolateral olfactory bulb and accessory olfactory bulb. Scale bar in f = 500 µm in b, c, e, and f.