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. 2021 Jun 18;12:3788. doi: 10.1038/s41467-021-24083-y

Fig. 1. RON13 is a RON kinase processed by ASP3.

Fig. 1

a U-ExM images of rhoptries from ASP3-iKD/RON13-3Ty extracellular parasites ± anhydrotetracycline (ATc). RON4 (green) and ROP2/3/4 (green) antibodies are used to visualize the neck and the bulb of the rhoptries, respectively. RON13-3Ty (magenta) is detected by anti-Ty antibodies. The subpellicular microtubules (gray) are stained with α/β anti-tubulin antibodies. Scale bar = 2 µm. Image representative of three biologically independent experiments. b 3D reconstruction from FIB-SEM images of the apical part of RH (control) and ASP3-iKD parasites treated 48 h with ATc. The neck (green) and the bulb (violet) of the rhoptries are colored. The conoid (black) and the PM (gray) are depicted. n = 1 biologically independent experiment. c Solubility of RON13 in ASP3-iKD/RON13-3Ty parasites ±ATc. Catalase is a marker of the soluble fraction. Samples derived from the same experiment and gels were processed in parallel. Image representative of three biologically independent experiments. d Scheme of RON13 protein and its cleavage by ASP3 just downstream the transmembrane domain (TMD). S/T serine/threonine kinase domain (orange), CTE C-terminal extension (gray). e Scheme representing the fate of RON13 in presence or in absence of ASP3. Without processing by ASP3, RON13 remains insoluble and mistargeted to the body of morphologically aberrant rhoptries. ELC endosome-like compartment. Source data are provided as a Source data file.