Fig. 6. Rhoptry proteins are the major substrate of RON13.

a Workflow of the shotgun approach and phosphoproteome analysis used to identify RON13 substrates. Samples were prepared for each strain from four independent experiments (n = 4 biologically independent experiments). RH (blue) RON13-KD (orange) RON13-KD/ron13wt (green) RON13-KD/ron13dk (salmon). b Venn diagrams of the phosphoproteins found in datasets 1 and 2. c Bar graph showing the percentage of phosphoproteins from datasets 1 and 2 relative to the total number of phosphoproteins, according to their predicted localization²³. EM endomembrane, IMC inner membrane complex. d Polar plot of the number of phosphopeptides found in both datasets (common) binned by gene IDs and clustered, according to their predicted subcellular localizations²³. Source data are provided as a Source data file.