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. 2021 Jun 18;12:3780. doi: 10.1038/s41467-021-24035-6

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — a Arginine methylation of immunoprecipitated S-Flag-SBP (SFB) tagged METTL14 protein detected by western blot in HeLa cells. MMA mono-methyl arginine, ADMA asymmetric dimethyl arginine. The experiment was performed once in HeLa cells. b Mass spectrometry showing that METTL14 R255 is methylated. The mass of each b ion and y ion are shown. The mass error of the peptide identification is 0.26326 ppm. c Arginine mono-methylation of S-Flag-SBP (SFB) tagged METTL14 and METTL14 R255K proteins analyzed by western blot. Representative figures of three independent replicates are shown. Source data for (a) and (c) are provided as a Source Data file. d Crystal structure ribbon diagram indicating the position of METTL14 R255 in the METTL3 (cyan) and METTL14 (pink) heterodimer. RNA is shown as a ball-and-stick model in the middle.