TABLE 1.

Summary of phenotypes detected in zebrafish embryos exposed to 0.25%, 0.5%, and 1% ethanol at 24 hpf, observations were made every 8 hr until the larval stage

Time (hpf)	Phenotype	Control (0%)	Eth 0.25%	Eth 0.5%	Eth 1%
8	Bud stage Visible somites	N Beginning	–	–	–
16	Spine shape Tail separating	N N	–	–	–
24	Eye formation Head separating Visible notochord Body movement Tail free Hatching	N N Low N – –	N N Low N – –	N N N N – –	N N N N Y Premature
32	Heartbeats Body movement Pigmentation Body reflexes Hatching	Y N N N –	Y N N N Y	Y Low N Low Y	Y Low N Low Y
40	Body mobility Abnormality	Low –	Low –	Low Yolk sac anomaly	Low Curved posture/pericardial edema
48	Heart shape	N	N	Pericardial edema	Heart edema
56	Tail shape Abnormality	N –	N –	Bent –	Bent –
64	Pectoral fin Abnormality	N –	N –	N –	N Delayed pigmentation
72	Swimming bladder Mandible Abnormality	N N –	N N –	N N Head in the eggshell	Minor enlargement N Head in the eggshell
80	Eye movement Startle response	N N	N N	N N	N N
88	Hatching Abnormality	Total –	Total –	Minor delayed/pericardial edema	Minor delayed –
96	Balanced posture Thigmotaxis	Mostly Mostly	Mostly Minor delayed	Mostly Minor delayed	Mostly Minor delayed
104	Body shape	N	N	N	Bent
112	Yolk sac Abnormality	Mostly absorbed –	Mostly absorbed –	Minor delayed –	Minor delayed Tail edema/heart edema
120	Body shape Swimming	N Y	N Y	N Y	Bent Y

N: normal, Y: yes, (–) any register.