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. 2021 Jun 14;16:4045–4061. doi: 10.2147/IJN.S305106

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© 2021 Petrini et al.

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DOX recovery from tumor (A) and endothelial cell lines (B) after incubation with 100 µM DOX either formulated in (C)TSLs or as a free form. DOX and protein content in cell lysates were assessed in cell lysate via HPLC and protein assay, respectively. Results are shown as DOX/protein ratio (µg/mg) and data are presented as mean value ± SD for three independent batches. Groups were analyzed via one-way ANOVA followed by Bonferroni test and asterisks indicate significant differences between groups. *** p < 0.001, **** p < 0.0001, LOD = limit of detection.