Table 1.
Characterization of Anionic and Cationic TSLs Encapsulating DOX
| Liposomes | Lipid Composition (mol:mol) | Size (nm) | PDI | ζ-POT (mV) | DOX/Lipid (mol:mol) | Tm (°C) |
|---|---|---|---|---|---|---|
| PG2-TSL-30 | DPPC/DSPC/DPPG2 (50/20/30) |
121.7 ± 5.4 | 0.06 ± 0.01 | −28.9 ± 5.4 | 0.056 ± 0.021 | 42.1 ± 0.4 |
| PG2-TSL-5 | DPPC/DSPC/DPPG2 (70/25/5) |
116.2 ± 3.3 | 0.07 ± 0.05 | −8.8 ± 2.4 | 0.049 ± 0.031 | 44.5 ± 0.2 |
| PEG-TSL-80 | DPPC/DSPC/DSPE-PEG2000 (80/15/5) |
111.1 ± 0.9 | 0.05 ± 0.02 | −4.7 ± 3.5 | 0.048 ± 0.016 | 43.0 ± 0.1 |
| PEG-TSL-70 | DPPC/DSPC/DSPE-PEG2000 (70/25/5) |
107.6 ± 0.8 | 0.06 ± 0.01 | −6.29 ± 3.0 | 0.049 ± 0.021 | 45.2 ± 0.2 |
| bare-CTSL | DPPC/DSPC/DPTAP (67.5/25/7.5) |
125.5 ± 5.4 | 0.19 ± 0.06 | 11.3 ± 3.6 | 0.048 ± 0.015 | 46.5 ± 0.2 |
| PG2-CTSL | DPPC/DSPC/DPTAP/DPPG2 (62.5/25/7.5/5) |
137.3 ± 5.1 | 0.29 ± 0.03 | 5.4 ± 2.3 | 0.046 ± 0.006 | 46.4 ± 0.4 |
| PEG-CTSL | DPPC/DSPC/DPTAP/DSPE-PEG2000 (62.5/25/7.5/5) |
110.9 ± 4.1 | 0.09 ± 0.07 | −0.2 ± 0.8 | 0.047 ± 0.007 | 46.3 ± 0.2 |
Notes: Ending numbers in DPPG2- and PEG-based anionic liposomes refer respectively to the DPPG2 and DPPC content. Results are shown as mean value ± SD for three independent batches.
Abbreviations: PDI, polydispersity index; ζ-POT, in 0.9% NaCl; Tm, melting temperature.