Fig. 2.

Shroom 2 Immunohistochemistry (upregulated on gene array at P90). (A) Retinal cross sections from Pde6b^rd1/rd1 and Pde6b^wt/wt mice stained for DAPI (blue), CHX10 (green) and Shroom2 (red). ONL – Outer nuclear layer, OPL – Outer Plexiform layer, INL- Inner nuclear layer, IPL –inner plexiform layer, GCL – Ganglion cell layer. Scale bar = 20 μm. White box indicates area from which close up images in panels B & D are taken. (B&D) Close up images of bipolar cell bodies in Pde6b^rd1/rd1 retina (B) and Pde6b^wt/wt (D) at four time points during degeneration (P40, P90, P120, P150). CHX10 (green) and shroom 2 (red). Scale bars = 5 μm. (C) Colocalised pixels above threshold - a semiquantitative index of protein staining. Normalised to highest value over all retinae stained for shroom 2. Dots represent the mean value for each animal, lines connect the means for each group at that timepoint. Star markers on graph represent adjusted p values, Sidak's method for multiple comparison (see results section for details): P90 p = 0.0136; P120 p = 0.0026 red line = Pde6b^rd1/rd1; blue line = Pde6b^wt/wt. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)