Fig. 3.

Sulphatase 2 Immunohistochemistry (upregulated on gene array at P90). (A) Retinal cross sections from Pde6brd1/rd1 and Pde6bwt/wt mice stained for DAPI (blue), PKC⍺ (green) and sulphtase 2 (red). ONL – Outer nuclear layer, OPL – Outer Plexiform layer, INL- Inner nuclear layer, IPL –inner plexiform layer, GCL – Ganglion cell layer. N.B. Due to primary antibodies being raised in differing species, to allow co-staining of sections, PKC⍺ is used as an ON-bipolar marker here, rather than CHX10. Scale bar = 20 μm. White box indicates area from which close up images in panels B & D are taken. (B&D) Close up images of bipolar cell bodies in Pde6brd1/rd1 retina (B) and Pde6bwt/wt (D) at four time points during degeneration (P40, P90, P120, P150). PKC⍺ (green) and Sulphatase 2 (red). Scale bars = 5 μm. (C) Colocalised pixels above threshold - a semiquantitative index of protein staining. Normalised to highest value over all retinae stained for Sulphatase 2. Dots represent the mean value for each animal, lines connect the means for each group at that timepoint. Star markers on graph represent adjusted p values, Sidak's method for multiple comparison (i.e. None reaching significance, see results section for details). red line = Pde6brd1/rd1; blue line = Pde6bwt/wt. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)